BOPI 10 BR/2023 REPERTOIRE NUMERIQUE 17 CUI, Qi (CN) et ZHAO, De (CN) (73) QINGDAO KINGAGROOT CHEMICAL COMPOUND CO., LTD., No. 53, Qinglonghe Road, Huangdao District, QINGDAO, Shandong 266000 (CN) (74) SPOOR & FISHER Inc. NGWAFOR & PARTNERS Sarl, The House of Gideon, Golf/Bastos Quarters, Opposite The American Embassy, Entrance-Saint John Paul II Boulevard, P.O. Box 8211, YAOUNDE (CM) (57) The present invention belongs to the technical field of pesticides, and in particular relates to a carboxylic acid derivative-substituted imino aryl compound, a preparation method therefor, a herbicidal composition and use thereof. The compound is as shown in general formula I: Q represents; Y represents halogen, haloalkyl or cyano; Z represents halogen; M represents CH or N; W represents OX5, SX5 or N(X5)2; X represents -CX1X2-(alkyl)n-, -alkyl-CX1X2-(alkyl)n-, or -(CH2)r-; X3 and X4 independently represent O, S, NH, Nalkyl, or the like. The compound has excellent herbicidal activity against gramineous weeds, broadleaf weeds, Cyperaceae weeds, and the like even at low application rates, and has high selectivity to crops. Formula I Consulter le mémoire ________________________________________ (11) 21191 (51) A01H 5/00 (2018.01); C12N 15/82 (2018.01) (21) 1202200178 - PCT/CN2020/126747 (22) 05/11/2020 (30) CN n° 201911073406.1 du 06/11/2019; CN n° 202011190279.6 du 30/10/2020 (54) Method for creating new gene in organism and use thereof. (72) CHEN, Bo (CN); JIANG, Linjian (CN); WANG, Jiyao (CN); MO, Sudong (CN) et LI, Huarong (CN) (73) QINGDAO KINGAGROOT CHEMICAL COMPOUND CO., LTD., No. 53, Qinglonghe Road, Huangdao District, QINGDAO, Shandong 266000 (CN) (74) SPOOR & FISHER Inc. NGWAFOR & PARTNERS Sarl, The House of Gideon, Golf/Bastos Quarters, Opposite The American Embassy, Entrance-Saint John Paul II Boulevard, P.O. Box 8211, YAOUNDE (CM) (57) The present invention relates to the technical fields of genetic engineering and bioinformatics, in particular, to a method for creating a new gene in an organism in the absence of an artificial DNA template, and a use thereof. The method comprises simultaneously generating DNA breaks at two or more different specific sites in the organism's genome, wherein the specific sites are genomic sites capable of separating different genetic elements or different protein domains, and the DNA breaks are ligated to each other through non-homologous end joining (NHEJ) or homologous repair to generate a new combination of the different gene elements or different protein domains that is different from the original genome sequence, thereby creating a new gene. The new gene of the invention can change the growth, development, resistance, yield and other traits of the organism, and has great value in application.
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